Journal: Stem Cell Research & Therapy
Article Title: Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing
doi: 10.1186/s13287-022-02918-9
Figure Lengend Snippet: Activin B-induced ADSCs proliferation by activating Cdc42-Erk-Srf pathway. A Genes expression related to MAPK signaling in Act B+ADSCs (Cdc42N17) group. B qPCR analysis was performed to confirm the relative mRNA expression level of Srf in four groups. C , E Western blot assays were performed to detect the level of Srf and phosphorylation Erk (p-Erk) in ADSCs, activin B+ADSCs, ADSCs (SCH772984) (Erk1/2 inhibitor) or ADSCs (Cdc42N17) treated with or without activin B, respectively. D , F The band and the relative quantification levels of the expression of Srf and phosphorylation Erk were analyzed. G Representative fluorescence imaging of EdU staining of ADSCs and ADSCs treated with Srf inhibitor CCG-100602 or Erk1/2 inhibitor SCH772984 and with/without activin B at 6 h. Scale bar = 50 μm. H The proliferation rates were quantified by calculating the percentage of EdU-positive cells. All values are expressed as mean ± SD from three independent repeats. * P < 0.05
Article Snippet: Cells were serum-starved for 12 h and then treated with serum-free H-DMEM containing Srf inhibitor CCG-100602 (15 μM, Cat# HY-120855, Mce) for 24 h [ , ] or Erk1/2 inhibitor SCH772984 (5 μM, Cat# S7101, Selleck) for 2 h [ ].
Techniques: Expressing, Western Blot, Fluorescence, Imaging, Staining